FastQCFastQC Report
Wed 25 May 2016
SRR1700069_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1700069_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8846569
Sequences flagged as poor quality0
Sequence length51
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGCGTT3809.485411E-723.86839145
GGTATCA85850.022.7165281
GTATCAA159850.020.3705961
TACCTGG65750.017.6647512
GTACCTG66600.017.6556191
TCAACGC198650.015.88503554
ATCAACG199300.015.8111143
ACCTGGG72200.015.6571943
CAACGCA204300.015.445735
TATCAAC207450.015.4044872
AACGCAG211900.014.9195786
GTACATG244050.014.110881
TACATGG243250.013.6084852
TTCGCGG4600.00546638313.14491145
ACATGGG247600.012.9670943
TAAACCG4702.845918E-712.86523345
ACGCAGA245350.012.8226487
GTATTAG25500.012.6311441
CGCAGAG250300.012.5866668
CGATCGT3409.094947E-1212.3114814
Produced by FastQC (version 0.11.5)