FastQCFastQC Report
Wed 25 May 2016
SRR1700068_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1700068_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6801483
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA53550.024.4025361
GTATCAA107650.022.296421
ATCAACG133100.017.5136553
TCAACGC133100.017.462574
CAACGCG2500.017.3705126
CAACGCA135100.017.1210275
TATCAAC142200.016.6455652
AACGCAG136750.016.5465286
TATACCG3300.015.6365315
AACGCGG2800.015.5093867
CGCGAAA1807.128983E-814.95825
AATCGCG3350.014.73279715
GTACATG168700.014.5337941
CGCAACG3300.014.27872120
CGCGCAA3000.014.20968318
ACGCAGA158750.014.1526847
TCGCGCA3500.014.10170317
TACATGG167600.013.9488812
CGCAGAG163750.013.6926258
GTATATA26200.013.5402681
Produced by FastQC (version 0.11.5)