FastQCFastQC Report
Wed 25 May 2016
SRR1700067_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1700067_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6926728
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACCTG58750.021.936671
TACCTGG57950.021.6423872
ACCTGGG64150.019.200943
GTATCAA168500.017.9620821
TATAACG4100.016.4166372
GGTATCA102000.015.7608471
GTACCGG5050.015.561211
ATCAACG199200.014.8560133
TCAACGC200450.014.7624114
CAACGCA202450.014.6276555
TATCAAC207800.014.2951662
AACGCAG203950.014.1206116
CCTGGGG51150.014.07924
ATAACGC4950.013.5976183
GTACATG176850.013.4322031
TACATGG174500.012.9859212
AACGCGG3700.012.9725177
GTATTAG19450.012.9290051
TAACGCA4550.012.8197784
GTATATA30850.012.8092641
Produced by FastQC (version 0.11.5)