FastQCFastQC Report
Wed 25 May 2016
SRR1700066_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1700066_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9565696
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTA144990.1515728703901943No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGCGA952.505062E-416.8399016
GTATCAA281150.016.0140461
CAACGCG3950.015.6218266
GTACCTG57850.014.1681861
TACCTGG55850.013.9395022
GGTATCA181100.013.7387131
GTACATG262950.013.5357041
ATCAACG326500.013.4545883
TCAACGC326850.013.4529954
GAGTACT155300.013.45054512
AGAGTAC288200.013.32233711
CAACGCA330300.013.25814155
TATCAAC337800.013.1439862
TACATGG265850.013.0217742
AACGCAG332150.012.95630556
GTATAGG21500.012.951141
GTATTAG24300.012.9373861
AGTACTT165850.012.75546513
TATACTG29950.012.7339615
GTATATA42000.012.4040481
Produced by FastQC (version 0.11.5)