FastQCFastQC Report
Wed 25 May 2016
SRR1700065_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1700065_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11739927
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAACGCG4200.019.7275186
GTATCAA262250.018.900881
GGTATCA153050.018.199111
TCAACGC315450.015.406594
ATCAACG317600.015.3156813
CAACGCA322200.015.0963145
TATCAAC330050.014.8058042
AACGCAG327950.014.5510996
GTACCTG63950.013.9461681
TACCTGG62700.013.9300952
GTACATG286750.013.9178721
TACATGG285400.013.5203612
AACGCGG6200.013.3638027
ACGCGGA6300.013.1516798
TATACTG36450.012.7177285
ACGCAGA373750.012.7020747
AGAGTAC342900.012.60998211
CGATCGT3203.092282E-1112.59612741
ACATGGG291650.012.5932813
TAGACCG2705.0276867E-912.4412555
Produced by FastQC (version 0.11.5)