FastQCFastQC Report
Wed 25 May 2016
SRR1700064_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1700064_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12223542
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTA295790.24198387014173142No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTA126190.10323521611002769No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACCTG79100.016.6847381
TACCTGG77700.016.0829722
ACCTGGG88200.013.7620723
GTACATG322550.013.5184351
TACATGG324650.013.1066392
CGCGCAA5050.012.8024518
GTATCAA416100.012.7246711
AGAGTAC348150.012.52309911
GTATTAA40400.012.42359351
GTATTAG36300.012.4070911
ACATGGG328150.012.4003983
TATACTG39650.012.3689995
GTATAGG24200.012.2219121
GTATATA46300.012.147121
CAACGCG4800.012.1300366
GAGTACT182900.012.12086912
GTATAAA43800.011.9707771
AGTACTT194850.011.59581413
CGCGGTA4300.011.45735143
GTATTAT29350.011.3751931
Produced by FastQC (version 0.11.5)