FastQCFastQC Report
Wed 25 May 2016
SRR1700063_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1700063_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8792258
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTA582250.6622303394645608No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTA268820.3057462599482408No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTT212210.2413600692791317No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT189730.2157921207498688No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC175540.19965292192290082No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCA137730.15664917931207206No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT130720.1486762558605537No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCA121220.13787129540557158No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT97680.11109774076238438No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGT96460.10971015636711298No Hit
GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAG89050.10128228721222694No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGCAA3650.014.17785318
GTACATG207600.013.9973071
CGCGATT2601.8189894E-1013.84537931
TACATGG207200.013.6069742
CGTTGCG2754.4019544E-1013.09159126
GTACCTG44150.012.9083851
ACATGGG210950.012.8210953
GTATTAG22200.012.6835011
TACCTGG42800.012.6699092
AGAGTAC214200.012.29959211
ATAATAC25700.012.1697563
AATCGCG4450.012.13401415
GAGTACT109900.012.01693412
TCTATAC20250.012.0004883
TATACCG4500.011.999675
GTAAGCG4900.011.93939726
GTATATA30700.011.73988151
TATATAC28400.011.7258293
TAAGCGG4800.011.71829327
TAGACTG18200.011.4969375
Produced by FastQC (version 0.11.5)