FastQCFastQC Report
Wed 25 May 2016
SRR1700062_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1700062_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13136376
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA266300.017.9403081
GGTATCA163650.017.3079221
CGCGCAA11000.014.69774718
ATCAACG320500.014.4917783
TCAACGC320800.014.4786674
TATCAAC332150.014.1587872
CAACGCA329400.014.1125365
ATCGCGC12200.013.94864216
GCGCAAC12000.013.85961619
GTACATG330900.013.8487451
ACGATCG4550.013.70736720
AACGCAG335450.013.6641126
TACATGG332750.013.4333232
TCGCGCA12200.013.39803717
AACGATA12300.013.3345123
CGCAACG12500.013.12048820
AATCGCG12700.012.87055615
GAGTACT169550.012.63840612
ACATGGG341650.012.6114383
GTATTAA38200.012.5534841
Produced by FastQC (version 0.11.5)