FastQCFastQC Report
Wed 25 May 2016
SRR1700061_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1700061_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5023208
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT51380.10228523286314245No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA100500.020.1938931
GGTATCA54700.020.0952681
GTACCTG30350.017.4409641
TACCTGG28350.017.3822842
TCAACGC118350.016.7866864
ATCAACG118800.016.7058243
CAACGCA120300.016.2714465
TATCAAC124100.016.1011522
AACGCAG122900.016.0723956
ACCTGGG31400.015.26390653
CGCGAAA1056.034727E-415.00234225
ACGATCG900.00375286315.00204620
CGCGCAA1502.576442E-615.000718
GTCGTAC1402.2098107E-514.4802411
ATCGCGA950.005372715614.21317123
TCGCGCA1901.3365207E-714.20906817
ACGCAGA143050.013.7769857
GAGTACT83050.013.68011212
GTACATG111900.013.5672281
CGCAGAG144750.013.5219278
Produced by FastQC (version 0.11.5)