FastQCFastQC Report
Wed 25 May 2016
SRR1700060_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1700060_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8041072
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA77250.024.1711251
GTATCAA151150.022.5312081
ATCAACG184650.017.949563
TCAACGC185300.017.9091034
TATCAAC193550.017.2636932
CAACGCA190650.017.2531245
AACGCAG197000.016.8105756
ACGCAGA228650.014.4245997
GTACCTG48050.014.0626991
GTACATG223850.014.0565961
CGCAGAG234750.014.0306068
TACCTGG46950.013.7066352
TACATGG225500.013.5504472
AGAGTAC233050.013.28343211
GAGTACT124200.013.22337212
TCTATAC16050.013.037923
ACATGGG228400.012.9646323
AGTACTT128050.012.86093313
CAGAGTA249550.012.79280610
GCAGAGT256200.012.6627249
Produced by FastQC (version 0.11.5)