FastQCFastQC Report
Wed 25 May 2016
SRR1700059_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1700059_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10578062
Sequences flagged as poor quality0
Sequence length51
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTN396380.3747189229936448No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTN182450.17247960921386166No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTN143520.13567702666140546No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCN125970.11908608590117925No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATN114580.10831851808015494No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG254600.013.0139861
GTATTAG28350.012.9167671
GTACCTG57450.012.9017331
TACACCG7100.012.7257875
TATACTG34100.012.5373735
GAGTACT140850.012.49781612
TACCTGG57150.012.4557552
TACATGG253000.012.3869072
CGAACTA4450.012.38005929
AGAGTAC272450.012.33182111
AGTACTT148700.011.71951813
ACATGGG257850.011.6917123
GTATATA42200.011.3957481
CAATCGA5302.3976463E-711.39386545
TCTATAC23300.011.3497023
GTACTTT152600.011.31893914
GTATTAA36950.011.283561
TAGACCG2751.3056924E-610.97954145
TATACGT5552.8414965E-510.88062845
GTACCGG6300.010.85475351
Produced by FastQC (version 0.11.5)