FastQCFastQC Report
Wed 25 May 2016
SRR1700058_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1700058_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9815366
Sequences flagged as poor quality0
Sequence length51
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTN125720.1280848824180372No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGCGTT3258.474672E-927.8212645
GTATCAA229200.016.0660021
TCAACGC267600.013.4658184
ATCAACG268800.013.4057713
CAACGCA270450.013.32391555
GGTATCA145100.013.2895451
AACGCAG277700.013.03517156
TATCAAC279950.012.9596932
TCTATAC24450.012.89022353
AGAGTAC252100.012.68088611
GTACATG238900.012.6523291
GAGTACT126000.012.58974612
TATACTG29700.012.3183555
TACATGG235050.012.1625622
ACGATCG2004.6415153E-612.11769320
TCCGGTC7554.810863E-811.9760445
AGTACTT132750.011.76702813
GTATTAG28550.011.6690881
ATACCGC4400.011.5167587
ATACCGT5750.011.4949876
Produced by FastQC (version 0.11.5)