FastQCFastQC Report
Wed 25 May 2016
SRR1700057_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1700057_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10359589
Sequences flagged as poor quality0
Sequence length51
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTN306380.2957453234872542No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTN144950.1399186782409997No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTN108840.1050620830614033No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGGGT5009.0474714E-818.13375945
GATCGGA3756.576419E-516.11889645
ATCGCGA2252.5465852E-1115.66824323
ACGATCG2254.2018655E-1014.68890820
CGCGCAA5450.014.14911318
TCGCGCA5500.014.02048417
AATCGCG5350.014.00176615
CGCGAAA2703.092282E-1113.87419625
GCGCGTT4900.013.491961531
GTATTAA28600.013.2665831
GTACATG268950.013.1643721
TATACTG31750.012.9092285
TCGCGAA2756.1299943E-1012.82052324
GTATTAG26400.012.7009541
TACATGG265800.012.6769562
GAGTACT120800.012.40222612
AGAGTAC264700.012.26877611
GCGCAAC6300.012.24034219
GTATATA36200.012.126661
AACGATA6850.011.90128923
Produced by FastQC (version 0.11.5)