FastQCFastQC Report
Wed 25 May 2016
SRR1700056_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1700056_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6621905
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA132500.016.8292621
GGTATCA84350.014.4825641
CGCGATT1758.4602834E-714.10153540
TCAACGC153350.014.0295344
ATCAACG153750.014.0226423
CAACGCA156750.013.7109145
GTACATG158000.013.5162151
AACGCAG156700.013.4185846
TATCAAC163050.013.3054792
TACATGG158650.012.9532952
GTATTAT19350.012.6496111
ACATGGG160600.012.4606553
GTATTAG20150.012.3702821
AGAGTAC150250.012.230082511
GCGCGTT2401.7838829E-712.15179831
ACGATCG1300.003086922712.08217420
GAGTACT70000.011.91743212
GTACCGT2502.3809844E-711.8847826
GTACCTG33600.011.7626471
CTAATAC18400.011.5831663
Produced by FastQC (version 0.11.5)