FastQCFastQC Report
Wed 25 May 2016
SRR1700055_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1700055_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6896346
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTA507680.7361579595919346No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTA246470.3573921610081629No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTT185320.2687220159777366No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT146710.2127358459102835No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC146090.21183681909231353No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCA127380.18470650979518718No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT110620.16040378484490192No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCA110270.15989626970572532No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT94050.1363765681130268No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGT86660.12566074846012656No Hit
ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCT78000.11310337387364265No Hit
GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAG77530.11242185354389121No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAACGCG3550.017.3774016
CGCGAAA2800.016.02796725
ACGATCG2900.015.47505520
ATCGCGA2800.015.22712223
TCGCGAA2950.014.45286124
TCGCGCA6200.014.11290617
GAGTACT124150.013.87631212
ATACCGC5450.013.8346087
GTACATG191050.013.7400721
AGAGTAC222350.013.61532911
CGCGCAA6100.013.609549518
ATCGCGC6950.013.23556816
TACATGG192100.013.163412
CGCATAG3101.6370905E-1113.02899920
AGTACTT132500.012.95327713
AACGATA6650.012.8228423
CGCAACG6500.012.77287220
AACGCGG4850.012.7195417
TATACTG19600.012.70577055
ATAATAC20250.012.6313143
Produced by FastQC (version 0.11.5)