FastQCFastQC Report
Wed 25 May 2016
SRR1700054_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1700054_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6456196
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA55250.024.26181
GTATCAA113000.022.3142491
TCAACGC140200.017.6500134
ATCAACG140600.017.6329423
CAACGCA143250.017.1802525
TATCAAC148000.016.8121932
TACCTGG40000.016.4347152
AACGCAG146750.016.3993726
GTACCTG41750.016.0265771
AATCGCG2453.6379788E-1215.56635515
ATCGCGA1056.1677897E-414.95938623
ACGCAGA166300.014.2515947
ACCTGGG44650.014.2204323
CGATCGT1901.3839417E-714.168756514
TCGCGAT2102.4401743E-814.14819411
CGCAGAG168650.014.0123558
TCTATAC16650.013.8794043
GTATTAG17700.013.8271981
TCGCGCA2601.891749E-1013.80588217
CGCGAAA1150.00122805813.65804125
Produced by FastQC (version 0.11.5)