FastQCFastQC Report
Wed 25 May 2016
SRR1700053_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1700053_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6769034
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTA128410.18970210520437628No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAACGCG2150.018.7465976
TACCTGG41650.017.2584152
GTACCTG42550.017.113871
AACGCGG2500.017.0177467
CGCATAG2700.016.5864968
ACCTGGG43650.015.5955343
ACGCGGA2853.6379788E-1214.142178
ATAACGC3500.014.0755553
GTATCAA207350.013.9503991
CGCGTAT1150.001247768413.629271512
GTATTAG20350.013.5426951
ACGCATA3350.013.3682227
CGCGGTA2205.8702426E-813.2323843
TAGACCG1709.897507E-613.1718245
GTACATG163500.012.8132191
TATAACG4050.012.7169722
GAGTACT108050.012.55799412
AGAGTAC188850.012.47300511
TACATGG161950.012.430532
ACGTCCG3254.0017767E-1112.40242143
Produced by FastQC (version 0.11.5)