FastQCFastQC Report
Wed 25 May 2016
SRR1700052_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1700052_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9286244
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTA100630.1083645874478422No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA217250.016.5942881
CGATCGT2252.0008883E-1115.92304614
GGTATCA138200.014.4130551
ATCAACG254550.013.8387883
TCAACGC255500.013.7965444
CAACGCA257600.013.6390615
TATCAAC263250.013.4580012
GTACATG212300.013.2556791
AACGCAG256200.013.2502116
GTACCTG49650.013.1321581
CAACGCG2951.05501385E-1012.9041576
TATACTG34500.012.7216775
CCGGTCG4050.012.7167329
TACATGG210950.012.66494752
AGAGTAC236700.012.648066511
CCGTTCG1608.1926155E-512.5957849
GTATATA40950.012.4203631
TACCTGG50150.012.3694752
CGCGGTA2553.0304363E-812.29418943
GAGTACT132650.012.20455712
Produced by FastQC (version 0.11.5)