FastQCFastQC Report
Wed 25 May 2016
SRR1700051_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1700051_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6660564
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTA89890.13495854104847577No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA174300.015.5734981
CCGATCG1859.643736E-814.593569
ATACCGT5500.013.9086
GTATTAG19350.013.7371791
GGTATCA114150.013.4982261
TCAACGC202050.012.972844
GTACATG170750.012.9684881
ATCAACG202800.012.9148373
GTCGTAC2451.5950718E-812.8723691
ATACCGC3355.456968E-1212.7602897
TATCAAC210250.012.649852
AACGCGG2857.767085E-1012.6305886
CAACGCA206150.012.6056925
AGAGTAC182150.012.58626111
TCTATAC16850.012.55254653
CCTATAC11300.012.5448663
AACGCAG209250.012.5151476
TATACTG21250.012.49375
TACATGG170850.012.406232
GTATATA29350.012.2802841
Produced by FastQC (version 0.11.5)