FastQCFastQC Report
Wed 25 May 2016
SRR1700050_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1700050_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9149987
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA210550.016.8254281
CAACGCG3150.014.2177596
TCAACGC244800.014.08931354
ATCAACG245150.014.0683533
GGTATCA134700.013.99000551
CAACGCA248850.013.8133515
CGCGCAA4250.013.585114518
TATCAAC258200.013.461392
GAGTACT117600.013.32842112
AACGCAG250900.013.2983366
CGATCGT3253.6379788E-1213.09048814
AGAGTAC227800.013.01434311
GTACATG212700.012.82072451
TATACTG32200.012.4487425
AGTACTT128350.012.43882313
TACATGG211600.012.340632
CGCGATT3551.4551915E-1111.98470940
ACATGGG213650.011.8972723
ACGCAGA281350.011.8352047
CGCAGAG285550.011.6532848
Produced by FastQC (version 0.11.5)