FastQCFastQC Report
Wed 25 May 2016
SRR1700049_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1700049_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4287216
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA49350.020.5851781
GTATCAA85500.020.2593481
CGTTCCG1354.283538E-818.33210434
TCAACGC104050.016.0014084
ATCGCGA1552.0673542E-715.96946423
ACGATCG1552.0689913E-715.96834720
ATCAACG105150.015.8362293
CGTATAC1451.8094997E-615.5189143
CAACGCA106500.015.4431665
CGCGAAA1754.8677975E-815.43005625
TATCAAC109750.015.3775122
AACGCAG109300.015.06743056
GTACATG103000.013.6462271
ATACCGT2652.4556357E-1013.5838356
TACATGG101100.013.221012
ACGCAGA124400.013.2023367
GAGTACT71200.012.98703912
ACATGGG100000.012.9163933
TCGCGAA2104.5100933E-712.85852924
CGGTAAT2805.857146E-1012.8574845
Produced by FastQC (version 0.11.5)