FastQCFastQC Report
Wed 25 May 2016
SRR1700048_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1700048_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4359439
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTA93770.21509648374481213No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACCTG34400.022.1335851
TACCTGG33400.021.9624672
ACCTGGG36900.019.4524483
CAACGCG1253.841851E-717.9988675
CGCGGTA1651.2332748E-917.72676743
GTACACG3700.014.6117761
ACGCGGA1901.3363024E-714.2091427
GTATTAG13150.014.0469361
CCTGGGG28750.014.0078144
ATAATAC16650.013.91983
ATAACGC3250.013.847033
AACGCGG1801.1255634E-613.7486616
GTACATG92450.013.7424981
GTATCAA133700.013.2934911
TAACGCA2754.4019544E-1013.0900864
TACATGG92550.013.0316182
GAGTACT71300.012.90547612
TATAACG3500.012.8579562
TACACGG4050.012.7785852
GTATTAT15200.012.5970491
Produced by FastQC (version 0.11.5)