FastQCFastQC Report
Wed 25 May 2016
SRR1700047_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1700047_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13163045
Sequences flagged as poor quality0
Sequence length51
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTN557330.4234050707871925No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTN262350.1993079868677802No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTN204280.15519205472593917No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCN182660.1387672837098103No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATN160630.122031034612432No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCN133030.10106324182588451No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGGTA5100.015.130280543
GTACCTG81450.015.0053751
TACCTGG77900.014.9103752
TCCGCAT12200.00403909914.83539945
CCGGACG8600.014.030375545
TTATACG8704.274625E-1013.86910745
ACCTGGG86200.013.1668153
GTACATG345450.013.098111
ATAACGC8750.012.845263
CGGACGT6050.012.74708636
GAGTACT196300.012.60423512
AGAGTAC373100.012.60161311
TACATGG344050.012.5943962
ATAATAC47050.012.5064023
TGCGCGA4907.4942363E-1012.31237145
TAACGCA6150.012.183854
TATAACG6950.012.0507542
AGTACTT204550.011.94508113
ACATGGG349750.011.9344663
CGCGCAA4650.011.84524618
Produced by FastQC (version 0.11.5)