FastQCFastQC Report
Wed 25 May 2016
SRR1700046_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1700046_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11536472
Sequences flagged as poor quality0
Sequence length51
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTN116740.10119211488572938No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA113900.021.0910991
GTATCAA216200.020.6310121
GTACCTG77450.018.0862031
TACCTGG75400.017.9178852
ATCAACG260200.016.6768283
TCAACGC261300.016.6571544
CAACGCA266400.016.3217225
TATCAAC272200.016.152972
AACGCAG276500.015.8170526
ACCTGGG85750.015.4202753
GTACATG284100.013.922081
ACGCAGA320450.013.6202277
CGCAGAG327400.013.317648
TACATGG283450.013.2803342
GTATTAG33600.013.0649821
AGAGTAC314400.013.06241811
GAGTACT172550.013.04956712
CGTTCCG3900.012.99516434
ACATGGG289100.012.5398633
GCGCGTT3900.012.43074931
Produced by FastQC (version 0.11.5)