FastQCFastQC Report
Wed 25 May 2016
SRR1700045_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1700045_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9982536
Sequences flagged as poor quality0
Sequence length51
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTN201120.20147185043960775No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACCTG61650.015.6716391
TACCTGG59600.015.56692
GTACATG271300.013.7488781
CGCGGTA4500.013.71922343
ACGTCCG5350.013.60016343
ACCTGGG67100.013.3665963
TACATGG270650.013.2315792
CGCGTAT1505.2103387E-513.21917712
GTATCAA300400.013.1219391
TCTATAC26100.012.9181363
ACATGGG274700.012.4744283
ATCGCGA2450.004768400512.25766545
GTATTAG26350.012.1383811
CGCAATA4950.001941644612.13384945
AGAGTAC275700.012.12277411
TAACGCA5350.011.9451924
TATACTG32750.011.8426885
TTAACGG4850.011.81219235
GTATATA37650.011.7175931
GAGTACT139350.011.69978712
Produced by FastQC (version 0.11.5)