FastQCFastQC Report
Wed 25 May 2016
SRR1700044_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1700044_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5765193
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTA73100.12679540823698357No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACCTG43500.018.630371
TACCTGG42300.018.6157322
ACCTGGG43000.017.4255943
GTATCAA136600.014.7416211
GTACATG126250.014.2964491
TACATGG125700.013.8139872
ACATGGG125200.013.27771953
ACGTCCG2401.2661985E-813.0863643
GTATATA26000.012.692511
GTATTAG17950.012.63165951
TCAACGC155750.012.574324
CGTTCCG1250.002304159812.56268834
ATCAACG155750.012.5458393
GTATAGG13650.012.4992641
CGGTCGT2206.557075E-712.467895510
CAACGCA157800.012.3683165
AGAGTAC134000.012.3500411
CCTGGGG35500.012.3226624
GTATTAT18000.012.2224171
TATCAAC163800.012.1211192
Produced by FastQC (version 0.11.5)