FastQCFastQC Report
Wed 25 May 2016
SRR1700043_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1700043_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8159991
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA171150.019.0899331
CAACGCG2550.017.9258756
GGTATCA98550.017.1348191
TCAACGC203700.015.51820854
ATCAACG204550.015.4541973
CAACGCA207400.015.2305475
AACGCAG208400.014.9262596
TATCAAC215700.014.7489472
ATAACGC5050.014.2165263
GTACATG197800.013.9749441
GTACCTG45350.013.3691811
TACATGG196500.013.3242642
AGAGTAC211250.013.22102211
GTATTAG25200.013.1880171
ACGCAGA234250.013.0839767
GAGTACT117450.013.03804112
GTATAGG20150.012.9271041
TATACTG26900.012.9270975
TAACGCA4350.012.8935464
TCTATAC20200.012.8837273
Produced by FastQC (version 0.11.5)