FastQCFastQC Report
Wed 25 May 2016
SRR1700042_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1700042_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9940487
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTA198550.19973870495479748No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGATCG2009.677024E-1015.70765220
GTACATG232400.013.9028721
CGCGCAA4650.013.5102618
TACATGG232450.013.4157662
GTATCAA297550.013.4093381
CGCAACG4700.012.89077920
AGAGTAC255300.012.83619811
GAGTACT142800.012.49858612
ATCGCGA2704.8748916E-912.46701723
CGTTCCG5050.012.43882634
ACATGGG240650.012.4179113
AGTACTT152600.012.0256713
TCTATAC25900.011.95389653
GCGCAAC5150.011.7635719
GTATTAG26400.011.7369441
CGCGGTA3451.10958354E-1011.70497543
GTATATA39850.011.6069671
GTACTTT159700.011.47698114
TCAACGC339950.011.4696934
ATCAACG339900.011.4651263
Produced by FastQC (version 0.11.5)