FastQCFastQC Report
Wed 25 May 2016
SRR1700041_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1700041_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11031061
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT114920.10417855544448534No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA123050.021.047531
GTATCAA229150.020.5707821
TCAACGC280050.016.4251964
ATCAACG280650.016.3654753
CAACGCG4250.016.333796
CAACGCA283700.016.2051735
TATCAAC294700.015.7068212
AACGCAG287750.015.5798396
ACGCGGA5150.013.47934158
ACGCAGA335400.013.3263647
GTACATG277050.013.1730951
GAGTACT190200.013.0916612
CGCAGAG341000.013.0418468
TATACTG35950.012.8942325
TACATGG277350.012.8218392
AGTACTT201150.012.712954513
GTACCTG63400.012.650821
AGAGTAC326450.012.64180511
TACCTGG60150.012.43482
TATACAG42700.012.2194625
Produced by FastQC (version 0.11.5)