FastQCFastQC Report
Wed 25 May 2016
SRR1700040_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1700040_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8038103
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTA81130.10093177457417503No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA198650.017.6903021
GGTATCA123350.015.2857071
ATCAACG237350.014.5799273
TCAACGC238400.014.5429414
CAACGCA241350.014.2253545
TATCAAC244850.014.2068452
AACGCAG245400.013.9534786
CGCGAAA2154.0758096E-813.60618625
GAGTACT132600.013.52250612
GTACATG200150.013.4834321
AGAGTAC233700.013.37167811
GTACCTG42200.013.291871
GTATTAG22500.013.2157131
AGTACTT139600.012.860558513
CGCGCAA3152.1827873E-1112.85739218
ACGTCCG3500.012.85675343
TACATGG202500.012.8001692
GTATTAT24300.012.792991
TACCTGG41050.012.7163682
GTATATA36150.012.6498971
Produced by FastQC (version 0.11.5)