FastQCFastQC Report
Wed 25 May 2016
SRR1700039_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1700039_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12504276
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTA216970.17351664342661663No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT141050.11280141289267767No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAACGCG4650.018.300046
GTATCAA391750.015.3631651
ACGATCG3000.014.62622120
GTACATG333400.013.6632971
ATCGCGA3550.013.66193523
CGATCGT4600.013.63031714
AACGCGG6450.013.5402377
TCGCGCA7550.013.3466217
TACATGG333150.013.2157292
GGTATCA262650.013.2061811
GAGTACT234700.013.03302212
AGAGTAC395500.012.72791611
ATCAACG465500.012.662333
TCAACGC465350.012.6477684
AGTACTT248550.012.63106413
TATCAAC475300.012.4719272
CAACGCA472550.012.4444375
ACATGGG343150.012.3998673
TATACTG40100.012.3975795
ACGCGGA6900.012.3326358
Produced by FastQC (version 0.11.5)