FastQCFastQC Report
Wed 25 May 2016
SRR1700038_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1700038_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1724240
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26260.15229898390015312No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17270.10016007052382497No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA37300.020.5969161
CCGGCCG600.00651554218.7481299
GGTATCA19450.018.5334841
TCAACGC44200.017.001554
GTACCTG11150.016.9730991
ATCAACG44450.016.9585133
TACCTGG10800.016.8762512
CAACGCA44600.016.6472855
ACACCGA952.8225302E-416.5772916
AACGCAG46300.016.2780726
TATCAAC47100.016.0521452
ACCTGGG11200.015.4698963
ATACCGT1056.043609E-414.9985026
TAGCGCA900.003757431914.9985019
GTACATG34650.014.4996761
ACGCAGA53450.014.1426477
AGAGTAC50800.014.12752611
TACATGG34100.013.9233192
GAGTACT35500.013.87889512
CGCAGAG54600.013.8035668
Produced by FastQC (version 0.11.5)