FastQCFastQC Report
Wed 25 May 2016
SRR1700037_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1700037_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5306960
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT56050.10561602122495742No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACCTG59350.027.7444931
TACCTGG58450.027.7195322
ACCTGGG61650.026.0982173
CCTGGGG45200.021.3048134
TAACGCA5350.019.7658984
GTATCAA108600.019.538951
TATAACG5150.019.2257522
ATAACGC5550.019.0564843
GGTATCA59150.018.470011
GTACACG3850.017.5525681
CGCGGTA2200.017.38579643
TCAACGC127000.016.2102534
ATCAACG127450.016.1554553
GTACCGG4450.015.6921261
CAACGCA130850.015.6645195
TACCCGG4800.015.4707242
TATCAAC134850.015.4524722
GTACCCG5250.015.44625951
AACGCAG135900.015.3624346
CTGGGGG25250.015.3263775
Produced by FastQC (version 0.11.5)