FastQCFastQC Report
Wed 25 May 2016
SRR1700036_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1700036_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9177892
Sequences flagged as poor quality0
Sequence length51
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTN210920.2298131204856191No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTN119490.1301932949309057No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTN96870.1055471125613594No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG78050.021.6868882
GTACCTG80950.021.4210531
ACCTGGG83600.019.4810123
CGAGACG4659.198642E-419.41620645
CGCGGTA3250.018.52007545
TAACGCA6900.018.2053554
ATAACGC7800.016.6698173
TATAACG7750.015.9255772
GTACACG5750.014.5797771
CCTGGGG71300.014.1871954
CGATCGT1952.3519169E-713.56624541
GTATCAA294850.013.4157291
GTATTAG26400.013.2034761
GTATAAC18950.012.806151
GAGTACT158500.012.73286112
CCGATCG2259.738869E-812.7297729
GTACATG230100.012.703821
AGAGTAC274350.012.56005611
TATACTG30500.012.50027755
GACGCTA4852.2001288E-412.41035945
Produced by FastQC (version 0.11.5)