FastQCFastQC Report
Wed 25 May 2016
SRR1700035_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1700035_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6349544
Sequences flagged as poor quality0
Sequence length51
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTN94320.14854610031838508No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTN71260.1122285316866849No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACCTG58100.023.2730241
TACCTGG57000.023.2381692
ACCTGGG60650.021.4024163
GGTATCA85850.018.9620171
GTATCAA146700.017.8630181
CCTGGGG52600.016.2982964
ATAACGC4750.015.7747913
TATAACG4800.014.6930192
TAACGCA5050.014.4012734
ATCAACG183000.014.0900683
TCAACGC184350.014.0107964
GTACCGG3800.013.9314431
CAACGCA187050.013.8085565
CGAGGCT6600.001986526213.65450945
TATCAAC190850.013.6151972
AACGCAG192950.013.4856096
GTATTAG19100.013.1655791
CCGATCG1353.170708E-413.0563479
CTTACGC2401.6241756E-812.8556823
TATACTG22650.012.5516215
Produced by FastQC (version 0.11.5)