FastQCFastQC Report
Wed 25 May 2016
SRR1700034_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1700034_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7066860
Sequences flagged as poor quality0
Sequence length51
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTN151060.2137583028388846No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGTAAT5155.091618E-429.0570145
CCCGCGA3959.903773E-422.73067545
AACGCGA4701.9434921E-519.1034445
TCGCGCA4650.017.53166617
GACCCGC5301.0320467E-416.94078445
AATCGCG4800.016.06575615
CGCGCAA4750.015.77101818
CGCAACG4900.015.28909620
CGTTCCG4400.015.0241234
GCGCGTT4700.015.0033431
ACGTCCG2000.00833499814.9643645
TAGGCCG4000.0082538814.9643645
ATCGCGA2652.1827873E-1114.135200523
GCGCAAC5800.013.67596919
AACGATA6300.013.64027223
ACGATCG2602.5283953E-1013.5595620
TACCGTC3101.8189894E-1213.504127
TAGACGT4457.1841685E-513.45111145
GTATCAA237150.013.3210521
CGCGAAA2857.6397555E-1113.143908525
Produced by FastQC (version 0.11.5)