FastQCFastQC Report
Wed 25 May 2016
SRR1700033_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1700033_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5444193
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTA198670.36492093502195827No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTA88810.16312794201087286No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTT67090.12323222192894337No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT60710.11151331335975782No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC57020.10473544931268969No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATCGT1257.854011E-616.15219714
GTATATA21750.014.7631461
CAACGCG2354.656613E-1014.5882236
TCGCGAT1604.2811716E-614.28430111
CCGATCG1108.7899936E-414.27593214
GTACATG129250.013.794061
CGTATCG1504.396654E-513.46016515
TACATGG128150.013.3063192
AGAGTAC147050.013.27308411
GAGTACT85800.013.21214812
ATTATAC16050.012.8607923
ACATGGG127650.012.8484733
ACGCGGA2703.7125574E-912.6971578
GTATAAT16000.012.6306211
TCGCACG1608.034803E-512.62180222
TTTCGCG1659.0223315E-512.46639
AGTACTT93500.012.40446613
AGCGCCG4000.012.34075225
TCTATAC14000.012.3400713
TATACTG19550.012.2786915
Produced by FastQC (version 0.11.5)