FastQCFastQC Report
Wed 25 May 2016
SRR1700032_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1700032_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5062504
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTA65090.12857273791783672No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT55740.11010361670825346No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACCTG42050.022.5370941
TACCTGG42250.021.7173082
ACCTGGG47000.018.7587593
CAACGCG2200.018.704386
GTATCAA146500.017.4290711
GTACACG3300.017.0128671
TATAACG4250.015.3080012
ATAACGC4050.014.9560933
CGATCGT900.003832247314.95491141
CCTGGGG38050.014.916054
TAACGCA4550.014.7910084
ACGCGGA2950.014.7239758
TAACGGC2152.6993803E-914.607409536
GGTATCA90400.014.4330851
ATCAACG174950.014.3491283
TCAACGC175950.014.3178694
TATCACG4550.014.298682
CAACGCA177500.014.1170115
TATCAAC179750.014.090762
AACGCAG178550.013.8151336
Produced by FastQC (version 0.11.5)