FastQCFastQC Report
Wed 25 May 2016
SRR1700031_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1700031_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8891178
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA121150.018.5878831
GTATCAA199800.018.4739071
ACGATCG2505.456968E-1215.25799420
ATCAACG241300.015.0248973
TCAACGC241750.014.987064
CAACGCA242550.014.8358855
CGCGAAA2457.2759576E-1114.65345325
AACGCAG244900.014.5315126
TATCAAC253650.014.3552622
CGCGCAA4950.014.050818
CAACGCG3850.013.6545726
GTACATG228350.013.21449851
AGAGTAC248200.013.15035311
TACATGG226650.013.0066992
GAGTACT137750.012.84281812
ACGCAGA275600.012.7718117
TCGTATG8450.012.74346640
GTATATA38400.012.4537691
CGCAGAG281750.012.4362438
ATCGCGA2901.0659278E-912.38012923
Produced by FastQC (version 0.11.5)