FastQCFastQC Report
Wed 25 May 2016
SRR1700030_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1700030_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9349017
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTA145770.15592013577470232No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT110800.11851513373010232No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAACGCG3850.018.0302946
GTACCTG58450.017.5573641
TACCTGG57150.017.280612
GTATCAA267000.015.4581211
ACCTGGG64550.015.0914073
AACGCGG4600.015.0905737
TCGCACG2651.6189006E-1013.94564318
ACGCGGA4750.013.6711888
GTACATG214100.013.1865831
GGTATCA177900.013.1367131
AGAGTAC276850.012.69809111
TCAACGC318250.012.6736174
ATCAACG319000.012.6502993
CAACGCA320650.012.5704295
TACATGG211500.012.53655052
TCTATAC24500.012.5225043
TATCAAC327500.012.3835122
GTATTAG26500.012.3448361
CGATCGT2003.87392E-612.31532814
CCGATCG2756.6156645E-912.21354814
Produced by FastQC (version 0.11.5)