FastQCFastQC Report
Wed 25 May 2016
SRR1700029_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1700029_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11961875
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA290700.017.4110131
TCAACGC340350.014.5230194
GGTATCA178650.014.5105261
ATCAACG340800.014.4765243
CAACGCG2950.014.4234226
CAACGCA346700.014.2558895
TATCAAC356850.014.0011372
AACGCAG349800.013.8667726
GTACATG273450.013.306411
GAGTACT203950.013.28523712
CGACGCT3500.013.19661521
TAGACCG3253.6379788E-1213.0923025
AGAGTAC331250.013.08751311
GCGTACG2307.018207E-813.05317421
TACATGG270800.012.826632
AGTACTT217150.012.78691813
ATCGCGA2551.970875E-812.6792123
ACGCAGA395550.012.2459337
ACATGGG272200.012.1765153
GTACTTT227150.012.11555114
Produced by FastQC (version 0.11.5)