FastQCFastQC Report
Wed 25 May 2016
SRR1700028_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1700028_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6582953
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT75170.11418887541806845No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACCTG47700.019.0305881
TACCTGG46700.018.7923372
GTATCAA164650.018.673961
ACCTGGG49700.017.2052233
GGTATCA94750.016.6411881
ATCAACG194300.015.4611433
TCAACGC194500.015.4198794
CGATCGT1502.58016E-614.99878514
TATCAAC202150.014.9609322
CAACGCA199300.014.8904535
AACGCAG204100.014.6938126
CAACGCG2451.0986696E-913.77512555
ATAACGC3600.013.7516013
AGAGTAC202900.013.73862311
AACGCGG3800.013.6173186
ACGCGGA4000.013.4991117
TAACGCA4200.013.3924844
GAGTACT130150.013.3452612
ACGCAGA229600.013.0424787
GTACACG4700.012.9399161
Produced by FastQC (version 0.11.5)