FastQCFastQC Report
Wed 25 May 2016
SRR1700027_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1700027_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8974500
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTA155340.17309042286478357No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT111020.12370605604769068No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACCTG60200.018.3121591
TACCTGG58700.018.0071452
TATAACG5650.016.6472242
ACCTGGG62750.016.4880493
TAACGCA5950.015.0558684
GTATCAA270600.014.8133571
ATAACGC6150.014.2013793
CAACGCG4150.014.02957256
AACGCCG4700.013.3407247
GTACATG201950.013.1364731
TTCGCGA2257.876224E-812.93838510
GTATTAG27250.012.8271171
TACATGG201950.012.7081342
GGTATCA182150.012.6208661
AGAGTAC271150.012.42040311
TATACTG31800.012.3235255
TCAACGC318100.012.2996294
GTACACG8200.012.2961631
ACATGGG201550.012.2666863
ATCAACG318550.012.2535173
Produced by FastQC (version 0.11.5)