FastQCFastQC Report
Wed 25 May 2016
SRR1700026_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1700026_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3178608
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT41500.13056029557592505No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA30100.022.7516691
GTATCAA62550.022.5811671
ATCGACG602.467893E-422.5012742
CGACGCA550.00393926320.4534514
ATCAACG77500.017.8848823
TCAACGC78000.017.7971254
TATCAAC80600.017.3924242
CAACGCA79850.017.3002645
AACGCAG81300.016.9358316
ACCGTCC1654.210615E-714.9987268
CGATCGT900.00375798714.99872614
ACGCAGA92100.014.9010147
GTACCTG18000.014.6426451
CGCAGAG94300.014.5772358
TACCTGG17800.014.4109292
TTCGCGA950.00538180914.20931810
GTACATG71250.013.9747331
AGAGTAC94500.013.87977211
GAGTACT63650.013.57308112
TCGCGAT1000.007559115513.49885311
Produced by FastQC (version 0.11.5)