FastQCFastQC Report
Wed 25 May 2016
SRR1700025_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1700025_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6436212
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTA245740.38180843017601035No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTA107120.16643329958677558No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTT82400.12802561506675045No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT79700.12383060098082536No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC71850.11163398595322839No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACCTG46250.022.1597941
TACCTGG46550.021.0751322
ACCTGGG50350.018.8589063
TATAACG4150.017.3502352
ATAACGC4150.014.6392613
CGATCGT1251.3830895E-414.39868414
TAGACCG2203.5815901E-914.3175385
CAACGCG2408.076313E-1014.0618695
GTACATG152200.013.9560681
GAGTACT106250.013.74216612
CGCGTAT1150.001203732113.69429212
TACATGG151700.013.6015152
AGAGTAC179750.013.26711111
TATACTG20500.013.1701415
CCTGGGG45200.012.9418974
GTATTAA22150.012.9150481
AGTACTT113150.012.86438813
TTCGCGA1403.6134987E-412.85586610
ACATGGG154600.012.7641993
TAACGCA4750.012.3152364
Produced by FastQC (version 0.11.5)