FastQCFastQC Report
Wed 25 May 2016
SRR1700024_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1700024_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4885574
Sequences flagged as poor quality0
Sequence length51
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTN565661.1578168706481573No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTN207040.4237782500070616No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCN170970.34994864472424325No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTN164350.3363985480518768No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATN144390.29554357379501367No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCN134300.27489093400284187No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTN113920.23317628593897055No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCN103130.21109085646845183No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAN86770.177604514843087No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGN84510.17297865102442414No Hit
GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAN83450.1708089980829274No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTN82260.16837325562973768No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTN69940.14315615729083214No Hit
AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCN68750.14072041483764242No Hit
ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCN64250.13150962404826944No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG39100.022.1543662
GTACCTG39950.022.0909181
ACCTGGG40700.021.0126513
TATAACG3350.017.7648872
TAACGCA3700.017.2758644
CCTGGGG34050.015.6006764
ATAACGC3750.014.6944123
TATACCG1606.228762E-613.7760115
TACGCAG4750.013.4569894
GTATAAC9700.013.4199491
CGACGCC1353.1666248E-413.05822433
GAGTACG7100.013.0515331
AGTACGC5250.013.0150522
GTACACG3750.012.9438061
CGCGAGT1250.002610378212.3554511
TACACGG3800.012.1808952
GACGCTA2453.70841E-612.17717545
TAGACCG1507.673665E-411.7555315
ACGTCCG2452.9250805E-711.69758943
GTACGCA5700.011.6008523
Produced by FastQC (version 0.11.5)