FastQCFastQC Report
Wed 25 May 2016
SRR1700023_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1700023_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7083186
Sequences flagged as poor quality0
Sequence length51
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTN107940.15238905204522374No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTN78570.1109246601741081No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA91000.020.7994671
GTATCAA167100.019.7761481
GTACCTG43900.016.683221
TACCTGG42150.016.6269452
ATCAACG204250.015.8172263
TCAACGC204600.015.7901684
CAACGCA208500.015.5159515
TATCAAC210800.015.4834382
CGACCGT1950.00680446415.35787245
TGCCGTC9801.5825208E-1015.27951545
TATAACG3900.015.2574552
AACGCAG214400.015.1369476
CGATCGT1402.7067175E-514.1723441
ACCTGGG48450.013.7818413
ACGCAGA245600.013.196077
TATACTG24300.013.0591415
GAGTACT147150.013.04121912
AGAGTAC235300.012.97781511
CGCAGAG249700.012.9705718
TTTCGCG1701.1683182E-512.96147236
Produced by FastQC (version 0.11.5)