FastQCFastQC Report
Wed 25 May 2016
SRR1700022_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1700022_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6291660
Sequences flagged as poor quality0
Sequence length51
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTN333150.5295104948455575No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTN137430.2184320195306167No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCN102960.1636452065114771No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTN101910.1619763305709463No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATN89650.14249021720817717No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCN84220.13385974448714646No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTN75660.12025443205767636No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCN69540.11052726943286827No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTN69420.11033654075395048No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGTTC3553.551486E-716.83152645
TATACTG18950.013.6075425
CGGACGT2250.002207160713.27820545
TACACGC4551.2291757E-513.1322945
GCGCGTT2704.5838533E-1013.05819931
GCGTACG1250.00263702412.33882221
TCGCGCA3601.8189894E-1212.24060217
CGCGCAA3451.2732926E-1112.13416218
GTACATG167850.012.0777881
TACACCG3703.6379788E-1211.9132785
GAGTACT118000.011.91280412
CGTTCCG3154.110916E-1011.8919134
GTACCTG31200.011.8781271
TACATGG168000.011.7813282
CCCGTCG2550.00682212111.71606345
CGCGATT1701.6689245E-411.67069440
AGAGTAC206650.011.62161311
AGTACTT120300.011.55683813
AATCGCG3704.3655746E-1111.31428615
TAGACAG15200.011.3097755
Produced by FastQC (version 0.11.5)