FastQCFastQC Report
Wed 25 May 2016
SRR1700021_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1700021_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1998714
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTA41810.20918450563712468No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT34940.17481240437601378No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22980.11497392823585566No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGACCGT702.3879365E-522.8651686
CAACGCG754.0674193E-521.3408246
GTACCTG12200.020.975361
CGCCGTA550.004002947420.39643925
CGCATAG806.685613E-520.0070238
TACCTGG12450.020.0012532
CGCGGTA707.1269495E-419.22852343
ACCTGGG12700.019.0775953
TAATCGG1104.7628324E-516.31388516
CCGGTCG1003.688188E-416.005629
GTACGGT1003.688188E-416.0056211
GACCGTG1155.914084E-515.9062047
GTATCAA67150.015.6445921
CAGTCCG900.00337365615.2434479
TGTACGG1055.3447916E-415.24344610
GTCGTAG900.003814227214.9648781
ACGCATA1251.2044556E-414.6337097
CGGTCGT950.004832904314.4411610
ACGCGGA950.004832904314.441168
TACGCTG950.005486651314.1676765
Produced by FastQC (version 0.11.5)