FastQCFastQC Report
Wed 25 May 2016
SRR1700020_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1700020_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5713750
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTA174960.30620870706628744No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTA83490.14612119886239333No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT79530.13919054911397943No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTT61070.10688252023627215No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAACGCG2450.021.460646
CGCGAAA1956.7666406E-1016.10895225
ACGCGGA3700.014.2104258
GAGTACT132650.014.20041212
ACGATCG1901.380995E-714.17115620
AACGCGG3750.014.0209537
AGTACTT137650.013.78758413
GTCGTAC1801.1518787E-613.7206731
AGAGTAC210100.013.72050211
GTATCAA253350.013.4437341
GTACATG156050.013.2510741
ATCGCGA2257.669951E-812.96443823
TACATGG155400.012.8770232
GTACTTT146450.012.8365614
ACGCATA2703.6998244E-912.7001387
TAATACC13200.012.5762694
CGCATAG2552.2711902E-812.5507258
ACATGGG157650.012.2805673
GTATTAA19150.012.1932581
ATAATAC17250.011.964663
Produced by FastQC (version 0.11.5)